SrasV1, Main, Exploration, bibRecord, 004E44

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Identifieur interne : 004E44 ( Main/Exploration ); précédent : 004E43; suivant : 004E45

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Auteurs : Yong-Hua Li [République populaire de Chine] ; JIE LI [République populaire de Chine] ; Xue-En Liu [République populaire de Chine] ; LING WANG [République populaire de Chine] ; TONG LI [République populaire de Chine] ; Yi-Hua Zhou [République populaire de Chine] ; HUI ZHUANG [République populaire de Chine]

Source :

Journal of virological methods [ 0166-0934 ] ; 2005.

RBID : Pascal:06-0005624

Descripteurs français

KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux, Anticorps monoclonaux, Dosage immunologique (), Femelle, Humains, Mâle, Protéines nucléocapside (immunologie), Protéines nucléocapside (sang), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (sang), Virus du SRAS (immunologie).
MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Protéines nucléocapside, Virus du SRAS.
- sang : Protéines nucléocapside, Syndrome respiratoire aigu sévère.
Pascal (Inist)
- Adulte, Adulte d'âge moyen, Anticorps antiviraux, Anticorps monoclonaux, Coronavirus, Dosage immunologique, Détection, Femelle, Humains, Mâle, Nucléocapside, Protéine, Sensibilité et spécificité, Sérum, Microbiologie, Méthode, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Adult, Antibodies, Monoclonal, Antibodies, Viral, Coronavirus, Detection, Female, Humans, Immunoassay (methods), Male, Method, Microbiology, Middle Aged, Nucleocapsid, Nucleocapsid Proteins (blood), Nucleocapsid Proteins (immunology), Protein, SARS Virus (immunology), Sensitivity and Specificity, Serum, Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (diagnosis), Severe acute respiratory syndrome, Virology.
MESH :
- chemical , blood : Nucleocapsid Proteins.
- chemical , immunology : Nucleocapsid Proteins.
- chemical : Antibodies, Monoclonal, Antibodies, Viral.
- blood : Severe Acute Respiratory Syndrome.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- methods : Immunoassay.
- Adult, Female, Humans, Male, Middle Aged, Sensitivity and Specificity.

Abstract

A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112769

Affiliations:

Le document en format XML

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<term>Antibodies, Monoclonal</term>
<term>Antibodies, Viral</term>
<term>Coronavirus</term>
<term>Detection</term>
<term>Female</term>
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<term>Microbiology</term>
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<term>Nucleocapsid Proteins (blood)</term>
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<term>Protein</term>
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<term>Sensitivity and Specificity</term>
<term>Serum</term>
<term>Severe Acute Respiratory Syndrome (blood)</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe acute respiratory syndrome</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
<term>Dosage immunologique ()</term>
<term>Femelle</term>
<term>Humains</term>
<term>Mâle</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines nucléocapside (sang)</term>
<term>Sensibilité et spécificité</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (sang)</term>
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</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Syndrome respiratoire aigu sévère</term>
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<term>Female</term>
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<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en">A capture enzyme-enhanced chemiluminescence immunoassay (ECLIA) based on three specific monoclonal antibodies to detect the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) associated coronavirus (SARS-CoV) in the serial serum samples from SARS patients was developed. The anti-SARS-CoV IgG and the viral RNA were also detected in the sera by ELISA and RT-PCR, respectively. During the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the N protein were detected in 21.4, 42.9, and 90% of the patients' sera, respectively. The detection rate of the N protein during days 11-15 of the disease was still significantly higher than those of anti-SARS-CoV IgG and SARS-CoV RNA. The data demonstrated that detection of the N protein with the capture ECLIA appears to be more useful than detection of other viral makers for rapid diagnosis of SARS in patients.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Yong Hua" sort="Li, Yong Hua" uniqKey="Li Y" first="Yong-Hua" last="Li">Yong-Hua Li</name>
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<name sortKey="Hui Zhuang" sort="Hui Zhuang" uniqKey="Hui Zhuang" last="Hui Zhuang">HUI ZHUANG</name>
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<name sortKey="Ling Wang" sort="Ling Wang" uniqKey="Ling Wang" last="Ling Wang">LING WANG</name>
<name sortKey="Liu, Xue En" sort="Liu, Xue En" uniqKey="Liu X" first="Xue-En" last="Liu">Xue-En Liu</name>
<name sortKey="Tong Li" sort="Tong Li" uniqKey="Tong Li" last="Tong Li">TONG LI</name>
<name sortKey="Zhou, Yi Hua" sort="Zhou, Yi Hua" uniqKey="Zhou Y" first="Yi-Hua" last="Zhou">Yi-Hua Zhou</name>
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   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
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   |clé=     Pascal:06-0005624
   |texte=   Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers
}}

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Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

Detection of the nucleocapsid protein of severe acute respiratory syndrome coronavirus in serum : Comparison with results of other viral markers

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